Increased Scrutiny of NGS Profiles / Ranking Does Not Improve eSet Implantation of Euploid Blastocysts: a Randomized Comparative Trial
Study question:
Can Next Generation sequencing (NGS) profile ranking of euploid embryos better predict eSET implantation potential of top quality blastocysts in contrast to morphological grading alone.
Summary answer:
When blastocysts are both top quality and euploid, the lack of noise on a NGS profile adds no benefit for eSET compared to embryo grading.
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Euploid Rate of Embryos Derived From Aspirated and Ejaculated Sperm
Objective:
To determine the euploid rate relationship between aspirated and ejaculated sperm-sourced embryos
Design:
Retrospective study
Materials and Methods:
Trophectoderm biopsy and PGS screening were performed on Day 5, 6 and 7 embryos were tested over a 2 year period. Biopsied embryos were segregated into based on sperm source: Percutaneous Epididymal Sperm Aspiration (PESA),
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An initial review of next generation screening: A new service provider for comprehensive chromosomal screening (CCS) by next generation sequencing (NGS)
Objective:
To review the initial results of embryo chromosomal competency and pregnancy success generated from one IVF laboratory by a new preimplantation genetic screening (PGS) service provider, Ovation Fertility Genetics.
Design:
Retrospective analysis in a private genetics laboratory.
Materials and Methods:
1861 blastocyst stage embryos underwent trophectoderm biopsy and subsequent comprehensive chromosomal screening (CCS) by next generation sequencing (NGS) over a 17-month period.
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Global multicenter randomized controlled trial comparing single embryo transfer with embryo selected by preimplantation genetic screening using next-generation sequencing versus morphologic assessment
OBJECTIVE:
The aim of this study was to assess the potential benefit of preimplantation genetic screening (PGS) across multiple international clinical sites and testing laboratories.
DESIGN:
Blinded, multicenter, randomized, controlled trial of women undergoing IVF with frozen embryo transfer: Control arm, embryo selection based on morphology; intervention arm, embryo selection by next-generation sequencing (NGS)-based PGS.
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The value in retaining non-pronuclear (0PN) zygotes for extended culture
Objective:
To determine the occurrence and ploidy status of usable embryos derived from oocytes not displaying two pronuclei (0PN) at the time of fertilization assessment.
Design:
Retrospective study in a private in vitro fertilization laboratory.
Materials and Methods:
A total of 3,370 blastocysts were identified as being morphologically suitable for fresh embryo transfer,
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Culturing embryos to day 7. A viable option for ivf patients?
OBJECTIVE:
To determine if culturing embryos to Day 7 improves IVF cycle outcomes.
DESIGN:
A retrospective analysis of 698 FET cycles was performed to evaluate the value of culturing embryos to Day 7.
MATERIALS AND METHODS:
Embryos were cultured in continuous culture medium (Irvine Scientific) from fertilization check through Day 7 without media renewal.
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Endometrial receptivity and pregnancy rates are higher after 7 days of progesterone in medicated FET cycles
OBJECTIVE:
To determine if Endometrial Receptivity Analysis (ERA) testing will improve implantation and pregnancy rates in patients undergoing medicated frozen embryo transfer (FET) cycles.
DESIGN:
Retrospective study in a large private practice fertility clinic.
MATERIALS AND METHODS:
We included all patients who had one or more ERA tests (Igenomix) in our clinic from 1/2016 to 5/2017.
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Examining the luteal placental shift in pregnancy after frozen embryo transfer
OBJECTIVE:
The purpose of this study is to attempt to define the timing of the luteal-placental shift using pregnancies resulting from frozen embryo transfer (FET) as a model.
DESIGN:
Retrospective chart review.
MATERIALS AND METHODS:
We identified 262 women who conceived following FET between December, 2013-December, 2016. Patients received oral estradiol (E2) for endometrial stimulation.
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Day 5, 6, and 7 blastocyst ploidy status stratified by patient age
OBJECTIVE:
To determine if a relationship of ploidy status in day 5, 6, and 7 blastocysts exists amongst patient age groups.
DESIGN:
Retrospective study in a private in vitro fertilization laboratory.
MATERIALS AND METHODS:
Blastocysts underwent trophectoderm biopsy and subsequent comprehensive chromosomal screening over the course of 3 years. PGS results were retrospectively compared to the day of trophectoderm biopsy and stratified by patient age.
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Blastocyst morphology is a poor indicator of euploid status
OBJECTIVE:
To evaluate the importance of blastocyst morphology in known euploid blastocysts.
DESIGN:
Retrospective study in a private assisted reproductive technology program.
MATERIALS AND METHODS:
The euploid status of blastocysts considered to be of marginal morphology was compared to that of good morphology blastocysts following trophectoderm biopsy and preimplantation genetic screening (PGS).
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Then and now: are past IVF experiences associated with current preferences regarding elective single embryo transfer?
OBJECTIVE:
Previous experiences with fertility treatment and pregnancy outcomes may influence the likelihood of a patient choosing elective single embryo transfer (eSET). Our objective was to investigate whether desire to undergo eSET is associated with previous IVF experiences.
DESIGN:
Retrospective descriptive analysis of online cross-sectional survey.
MATERIALS AND METHODS:
An anonymous survey regarding eSET preferences and experiences was distributed through social media over a five-week span in 2014.
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Demographic characteristics associated with elective single embryo transfer in IVF: who chooses just one?
OBJECTIVE:
Younger patient age has been found in previous IVF studies to be associated both with desire for twins and the choice of single embryo transfer; we sought to examine this and other demographic and geographic parameters in relation to elective single embryo transfer (eSET).
DESIGN:
Descriptive analysis of online survey results from a cross-sectional sample of U.S.
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